Plasmid
recombinant plasmid
recombinant plasmid
Plasmid VRD2E
Construct Insert
Construct insert
has part construct insert
construct insert
has_part_construct_insert
prM/env
construct insert
prM/env
Dengue virus
processed material
processed material
material_entity
independent_continuant
continuant
entity
Organism Expression Target
expressed in organism
expressed_in_organism
Homo sapiens
Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs.
Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.
Resource Description
resource_description
Vical pVR1020 vector
Construct Backbone
construct backbone
construct_backbone
Manufacturer
has manufacturer
has_manufacturer
MartĂnez, IdalĂ
Contact
has contact
has_contact
Kanamycin
Selectable Marker
has selectable marker
has_selectable_marker
Location
located_in
Idali Martinez Laboratory
located in
2010-08-31T19:20:06.480-05:00
mbrush
2016-08-26T15:19:20.516-05:00
workflow state
Published
tjohnson
Construct
construct
construct
Reagent
reagent
reagent
processed material
processed material
material_entity
independent_continuant
continuant
entity