Plasmid VRD3E

eagle-i ID

Resource Type

Properties

1. Construct Insert

   prM/env

2. Resource Description

   Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs. Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.

3. Manufacturer

   Martínez, Idalí

4. Construct Backbone

   Vical pVR1020 vector

5. Selectable Marker

   Kanamycin

6. Organism Expression Target

   Homo sapiens

7. Location

   Idali Martinez Laboratory