Fluorescence recovery after photobleaching protocol

eagle-i ID

Resource Type

Properties

1. Resource Description

   "Fluorescence recovery after photobleaching (FRAP) denotes an optical technique capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding. In addition, surface deposition of a fluorescing phospholipid bilayer (or monolayer) allows the characterization of hydrophilic (or hydrophobic) surfaces in terms of surface structure and free energy. Similar, though less well known, techniques have been developed to investigate the 3-dimensional diffusion and binding of molecules inside the cell; they are also referred to as FRAP." Reference: http://en.wikipedia.org/wiki/Fluorescence_recovery_after_photobleaching

2. Additional Name

   FRAP protocol

3. Uses

   Fluorescence recovery after photobleaching

4. Uses

   Zeiss Axiovert 200 confocal microscope

5. Uses

   Incubator INU-ZILCS-F1

6. Used by

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