Center for Genomics in Health Disparities and Rare Disorders

People:

• PI: Rodriguez Medina, Jose, Ph.D.
  

Member: Villanueva, Lilliam, M.S.
Role: Laboratory Technician

Resources:

Instruments

• ABI 3130 Genetic Analyzer ( Multicapillary electrophoresis system )
  

  "The Applied Biosystems® 3130 Genetic Analyzer is the latest generation of 4-capillary electrophoresis instruments for the low to medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity.
  
  With the Applied Biosystems® 3130 Genetic Analyzer, you get the sophisticated automation and superior performance of the 3130 system, at acquisition and operating costs tailored to a growing research lab.
  • Run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening.
  • Reduce maintenance time by eliminating manual syringe washing and filling with automated polymer delivery.
  • Increase your data quality for sequencing and fragment analysis applications – longer read length, and higher resolution with shorter run times.
  • Increase laboratory productivity and turn around time by processing 96- or 384-well plates with a four-capillary array. "

• ABI 3400 DNA synthetizer ( Oligonucleotide synthesizer )
  

  A-621

• ABI Veriti thermal cycler ( Thermal cycler )
  

  A-633

• Bio-Rad 680 ELISA reader ( ELISA microplate reader )
  

  A-634

• Bio-Rad Laboratories, Inc. Gel Doc 1000 Fluorescent Imaging System ( Gel imaging system )
  

  A-624
  
  "To capture images and perform densitometric measurements on autorads, x-ray films, autoradiograms, SDS-page gels, silver-stained gels (wet or dry), polaroids, blots on nitrocellulose, ethidium bromide gels."

• Bio-Rad Laboratories, Inc. Molecular Imager PharosFX Plus System ( Gel imaging system )
  

  The Molecular Imager PharosFX Plus System "can be used to image many types of samples (such as gels, blots, and microplates) and to support a wide range of imaging applications."

• Bio-Rad VersArray chip reader with Dell computer and ImaGene software for microarray data analysis ( Array scanner )
  

  A-634

• BioRad iCycler thermal cycler ( Thermal cycler )
  

  A-633

• BioRad Protean IEF cell system ( Gel electrophoresis system )
  

  A-634

• Branson Ultrasonic 2510 sonicator ( Sonicator )
  

  A-634

• iCycler Real-Time PCR unit ( Real-time PCR machine )
  

  A-633; 2 RT-PCR machines

• Ion Torrent PGM Semiconductor Sequencer ( DNA sequencer )
  

  "The new Personal Genome Machine (PGM™) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any lab or clinic. The system’s use of the simplest, natural sequencing chemistry eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension."

• Lab line incubator oven ( Incubator )
  

  A-634

• Millipore Milli-Q water system ( Water purification system )
  

  A-621

• Nanodrop 1000 spectrophotometer ( Spectrophotometer )
  

  A-634

• Nanodrop 8000 spectrophotometer ( Spectrophotometer )
  

  A-634

• NuAire biological hood ( Biological safety cabinet )
  

  A-634

• Olympus fluorescence microscope with spot monochrome camera ( Fluorescence microscope )
  

  A-633

• Pharmacia PhastSystem ( Electrophoresis system )
  

  A-621

• Refrigerated centrifuge ( Refrigerated centrifuge )
  

  GS-15R Refrigerated Centrifuge with rotors for 1.5, 2.0, 15, and 50 mLs tubes.

• Savant sample concentrator ( Vacuum centrifuge concentrator )
  

  A-633

• Savant sample concentrator for DNA Samples ( Vacuum centrifuge concentrator )
  

• Shel Lab hybrididation chamber ( Hybridization chamber )
  

  A-634

• Spectrophotometer ( Spectrophotometer )
  

• VirTis Virsonic ultrasonic cell disrupter ( Ultrasonic cell disruptor )
  

  A-633

Protocols

• DNA sequencing protocol ( Protocol )
  

• Oligonucleotide synthesis protocol ( Protocol )
  

Services

• Automated DNA sequencing ( Material analysis service )
  

  Service fees apply. Contact lab for details.

• Oligonucleotide synthesis service ( Material production service )
  

  Service fees apply. Contact lab for details.

Web Links:

• http://rcmi.rcm.upr.edu/resreso/corefacnetwork/cghdrd