Idali Martinez Laboratory

People:

• PI: Martínez, Idalí
  

Resources:

Instruments

• Beckman Coulter Allegra 6R centrifuge ( Refrigerated centrifuge )
  

  "The Allegra 6 Series is designed for high-volume labs requiring a simple, durable solution above all else. Save time with the basic, streamlined user interface that is easy to use and quick to program. Available in refrigerated and ambient benchtop and refrigerated knee-well models."

• BioRad iCycler Thermal cycler ( Thermal cycler )
  

• Microfuge centrifuge ( Microcentrifuge )
  

• NuAire Incubator ( Incubator )
  

  Laboratory has four incubators.

• PCR workstation ( PCR instrument )
  

  With model 300 controller.

• Shaker ( Orbital shaker )
  

• Temperature controlled water bath ( Water bath )
  

  Lab owns 2 of these instruments.

• Thermo Fisher Scientific Locator Jr cryobiological storage system ( Liquid nitrogen dewar )
  

• UVP M-20 UV transilluminator ( Ultraviolet transilluminator )
  

Reagents

• Plasmid VRD1E ( Plasmid )
  

  Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs.
  
  Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.

• Plasmid VRD2E ( Plasmid )
  

  Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs.
  
  Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.

• Plasmid VRD3E ( Plasmid )
  

  Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs.
  
  Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.

• Plasmid VRD4E ( Plasmid )
  

  Plasmid was derived from the eukaryotic expression vector pVR1020, provided by Vical, Inc. (San Diego, CA). This vector contains the cytomegalovirus (CMV) immediate early promoter, including the intron A, the bovine growth hormone polyadenylation signal, and the tissue plasminogen activator (tpa) leader sequence. The tpa leader sequence was not included in the final constructs.
  
  Organism expression target: The recombinant prM/E proteins from Dengue virus can be expressed in eukaryotic cells. COS-7 and 293T cells were used to test the in vitro expression of these proteins.

• VR1020 ( Expression construct )
  

  Parental vector (VR1020) provided by Vical through MTA.