"Our laboratory investigates the cellular and behavioral changes in response to exposure to natural and synthetic androgens. We are currently studying effects of anabolic androgenic steroids (AAS) on anxiety, reproductive behaviors and energy metabolism. Cellular substrates such as neuropeptides and neurotransmitters present in the hypothalamus and amygdala are possible candidates for modulation by AAS. Experiments are done both in vivo and in vitro. Results will provide novel information to understand the behavioral consequences of AAS induce cellular regulation from adolescents to adults." (http://www.md.rcm.upr.edu/anatomyneurobiology/jennifer_barreto_estrada.php)
Inmortalized hypothalamic tumor cell line provided by Dr. Pamela Mellon in agreement of biological materials between Salk Institute, La Jolla, CA and University of Puerto Rico, Medical Sciences Campus.