The Translational Proteomics Center provides training, resources, and tools for new and established investigators seeking to use innovative approaches of proteomics to their research, as well as to undergraduate and graduate students in the biomedical field. The Center fosters collaborations and partnerships among researchers aiming to reduce health disparities, including infectious diseases, cancer, and neurological/neurodegenerative diseases.
Currently located at the second floor of the Comprehensive Cancer Center, Laboratory # 5.
Ettan IPG Phor 3 & manifold
Ettan Dalt TwelveSeparation Unit
Ettan DIGE Imager
Ettan Spot Picker & Base
"The Gel Doc™ XR+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and densitometry. The system is controlled by Image Lab™ software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.
The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose™ gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization."
(purchase in process)
Bio Med #1 Lab 302
"The SmartSpec Plus spectrophotometer has a wider range of features and functions than many other benchtop spectrophotometers, offering performance, stability, and functionality at an affordable price.
The UV/visible SmartSpec Plus spectrophotomer has a working wavelength range of 200–800 nm. It is the perfect tool for routine applications such as:
Quantitation of DNA, RNA, and oligonucleotides
Quantitation of proteins via the Bradford, Lowry, and BCA assay methods
Monitoring bacterial culture growth
Simple kinetic assays
Wavelength scans with peak detection"
Micro 17 and Micro 21R
Thermo Finnigan Surveyor MS Pumps
Thermo Finnigan Micro AutoSampler
Eksigent Nano LC
"The LTQ XL extends the legendary MSn performance of the LTQ, incorporating more techniques to generate structural information through innovations in Thermo Scientific ion trap technology.
Unparalleled sensitivity and unmatched MSn spectral quality
Pulsed Q Dissociation (PQD) for generating more fragments and extending the low mass range
Electron Transfer Dissociation (ETD) option provides sequence information not available from conventional methods
High Resolution Isolation (HRI) enables isolation of isobaric mass peaks for cleaner MSn spectra
Ultra-fast polarity switching for high sensitivity analysis of unknowns
Automated Data Dependent neutral loss for PTM and biotransformation analyses
Excellent charge state determinations with Ultra ZoomScan™
Upgradeable to LTQ Orbitrap XL accurate mass technology"
Use techniques such as: Itraq, SILAC and others.
Itraq, ICAT, SILAC, enzymatic digestion of samples (in gel, in solution or in beads), C18 zip-tip sample clean up and MS/MS analysis.
Consulting about proteomics, platforms, experimental design, support letters, methods preparation for proposals, budgets, results analysis and reports preparation.
Includes: statistics, database search and pathways elucidation using Proteom Discoverer, Mascot, Scaf fold and Ingenuity Pathway Analysis (IPA).
"2D gels differential analysis software specifically designed for 2-D fluorescence (DIGE) analysis using the Ettan DIGE system, multivariate statistics module for biomarker discovery"
Nano LC control software
Data analysis, statistics and graphics
"Imaging software and toolbox for area and profile analysis of acquired images, software contains several independent modules, which may be run simultaneously."
Imaging and data analysis software.
"Database search engine tool for the analysis of protein sequencing data"
"Database search engine tool for the analysis of protein sequencing data from Thermo Scientific"
"Database search engine tool for the analysis of protein sequencing data, including quantitative proteomics methods"
MS/MS Spectrometer control software
LTQ MS/MS data acquisition and control software.