Carlos A. Jiménez-Rivera Laboratory

People:

• PI: Jiménez-Rivera, Carlos
  

Resources:

Instruments

• AccuScan MKRO16 animal activity monitor system ( Animal activity monitoring system )
  

  Instrument uses infrared.

• Axon Axopatch 1D amplifier ( Analog-to-digital converter )
  

  The Axopath 1D features a lag control to provide stability and maintain current recording bandwidth. The Axopath ID also has a resistor-feedback technology for whole-cell, single-channel recording.

• Axon Axopatch 200B Capacitor feedback patch clamp amplifier ( Analog-to-digital converter )
  

  "Widely accepted as the gold-standard for ultra low-noise patch clamp recordings, the Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier is the benchmark for single-channel recordings and other applications that require an excellent signal-to-noise ratio. By introducing active cooling of components in the headstage to well below 0°C, the open-circuit noise in patch mode has been reduced to unprecedented levels, as low as <15 fA (RMS) at 0.1 - 1 kHz. This translates into significantly lower noise during patch clamp recordings. A headstage that can be switched to resistive mode ensures superb performance in whole-cell recordings, too."

• Axon Multiclamp 700B microelectrode amplifier ( Analog-to-digital converter )
  

  "The Multiclamp™ 700B Microelectrode Amplifier combines leading edge electronics design and a wealth of features and functions with an intuitive user interface that simplifies the creation of complex protocols. The applications for this amplifier comprise a wide variety of intracellular or extracellular recordings in electrophysiology, including:
  *Patch clamp (whole-cell, macro-patch, or excised patch)
  *High-speed current clamp (sharp-electrode or field potentials)
  *Voltammetry/amperometry
  *Ion-selective measurements
  *Bilayer recordings."

• BH-2 Micro-iontophoresis device ( Micro-iontophoresis device )
  

• Bio Rad Ultra Rocker ( Rocker )
  

• DAGE-MTI IR1000 digital camera ( Microscope digital camera )
  

• Hitachi oscilloscope ( Oscilloscope )
  

• KD Scientific 74900 Syringe pump ( Syringe pump )
  

• Leica VT1000S Vibrotome ( Vibratome )
  

• Micro-iontophoresis device ( Micro-iontophoresis device )
  

• Narishige PE2 Electrode puller ( Electrode puller )
  

• Narishige PP830 Micropipette puller ( Micropipette puller )
  

• Olympus BX51WI Infrared microscope ( Infrared microscope )
  

• Olympus BX51WI Infrared microscope ( Infrared microscope )
  

• Olympus BX51WI Infrared microscope ( Infrared microscope )
  

• Plexon Multichannel Acquisition Processor 16 channel system ( Multichannel acquisition system )
  

• SD Instruments MX7500L Micromanipulator ( Micromanipulator )
  

• Single stereotaxic device ( Single stereotaxic device )
  

  Lab contains two of these instruments.

• Stimulus isolator ( Stimulus isolator )
  

• Stoelting Co. dual stereotaxic device ( Dual stereotaxic device )
  

• TAS 250 oscilloscope ( Oscilloscope )
  

  Two channels. Two copies of this resource available.

• TMC Vibration isolation table ( Vibration isolation table )
  

  5 vibration isolation tables

• Vibration isolator table ( Vibration isolation table )
  

• Whisper Room SE2000 sound isolation enclosure ( Sound isolation enclosure )
  

• World Precision Instrument A365 stimulus isolator ( Stimulus isolator )
  

• WPI A310 Accupulser ( Simulator )
  

  Lab contains 2 instruments.

Protocols

• Behavioral sensitization to cocaine in rats protocol ( Protocol )
  

• Brain cannula surgery protocol ( Protocol )
  

  Microinjection system

• In vivo extracellular electrophysiology recording protocol ( Protocol )
  

• Intracellular electrophysiology recording protocol ( Protocol )
  

  Whole cell patch clamp.

Software

• Micromax ( Software )
  

  Used for animal activity monitoring.

• pCLAMP ( Software )
  

  Electrophysiology data acquisition and analysis software. Two copies of this software available.

• Spike2 ( Software )
  

  Life sciences data acquisition and analysis system.