Pharmacology / Molecular Biology Laboratory

People:

Member: Pérez, Marianela, M.T., Ph.D.

Resources:

Instruments

• Barnstead 460 CO2 incubator ( CO2 incubator )
  

• Fisher Scientific 100 sonicator ( Sonicator )
  

• Motic AE20 Inverted microscope ( Inverted microscope )
  

• NuAire NU-425-400 Biological safety cabinet ( Biological safety cabinet )
  

• SNAP i.d. protein detection system ( Protein separation apparatus )
  

• Tomy MX-305 Centrifuge ( Centrifuge )
  

Organisms and Viruses

• Primate ( Primates )
  

Reagents

• A431 ( Tumor-derived cell line )
  

  Vulvar carcinoma.

• BeWO ( Tumor-derived cell line )
  

  Placenta cell line.

• BT-20 ( Tumor-derived cell line )
  

  Breast adenocarcinoma.

• CaOv3 ( Tumor-derived cell line )
  

  Derives from ovarian epithelial adenocarcinoma.

• CHO/EGFR ( Tumor-derived cell line )
  

• CHO/ErBb2 ( Tumor-derived cell line )
  

• CHOpcDNA 2241 (p110) ( Tumor-derived cell line )
  

• COS-1 ( Cell line )
  

• Detroit 562 ( Tumor-derived cell line )
  

  Pharynx epithelial carcinoma.

• Drosophila S2 ( Cell line )
  

  Macrophage-like lineage cells.

• HeLa ( Tumor-derived cell line )
  

  Derived from epithelial-cervix adenocarcinoma.

• Hs578Bst ( Tumor-derived cell line )
  

  Derived from breast-epithelial adenocarcinoma.

• HUVEC ( Cell line )
  

  Endothelial cells from the umbilical vein.

• JAR ( Tumor-derived cell line )
  

  Derives from placenta/choriocarcinoma cell line.

• JEG-3 ( Tumor-derived cell line )
  

  Derives from placenta/choriocarcinoma cell line.

• MAb-528 ( Hybridoma cell line )
  

  Produces the monoclonal antibody 528, which recognizes the epidermal growth factor receptor.

• MCF12A ( Tumor-derived cell line )
  

• MCF7 ( Tumor-derived cell line )
  

  Derives from breast epithelial adenocarcinoma

• MCF7/2241 (p110) ( Tumor-derived cell line )
  

  Derived from breast epithelial adenocarcinoma.

• MDA-MB-231 ( Tumor-derived cell line )
  

  Derived from breast epithelial adenocarcinoma.

• MDA-MB-435 ( Tumor-derived cell line )
  

  Derived from breast epithelial adenocarcinoma.

• MDA-MB-468 ( Tumor-derived cell line )
  

  Derived from breast epithelial adenocarcinoma.

• pcDNA 2241 (p110) ( Expression construct )
  

• pcDNA-EGFR ( Expression construct )
  

• pcDNA-ErbB2 ( Plasmid )
  

• pcDNA-p100-sEGFR ( Plasmid )
  

• pcDNA-p60-sEGFR ( Plasmid )
  

• QT6 ( Cell line )
  

  Derived from fibrosarcoma.

• QT6/2241 (p110) ( Cell line )
  

  Derived from fibrosarcoma.
  
  Stably transfected with the pcDNA vector containing the cDNA of p110-sEGFR.

• QT6pDR161 ( Cell line )
  

  Derived from fibrosarcoma.

• SK-BR-3 ( Tumor-derived cell line )
  

  Derives from breast epithelial adenocarcinoma.

• T47D ( Tumor-derived cell line )
  

  Derives from breast adenocarcinoma.

• ZR-75-1 ( Tumor-derived cell line )
  

  Derives from breast epithelial adenocarcinoma.

Web Links:

• http://farmacia.rcm.upr.edu/SchoolResearch.htm