Virology Laboratory

Summary:

Research interests include: immunogenicity and protective efficacy of multi-clade consensus ENV SHIV virus-like particle vaccine, cellular innate activation as a tactic to prevent HIV-1 transmission, molecular changes after serial DV infections in macaques, and immunization of rhesus macaques with a dengue vaccine candidate based on a Venezuelan Equine Encephalitis (VEE) replicon vector.

People:

• PI: Kraiselburd, Edmundo
  

Member: Sariol Curbelo, Carlos
Role: Director

Resources:

Instruments

• BD FACSAria I flow cytometer ( Flow cytometer )
  

  "The BD FACSAria flow cytometer is the first fixed-alignment benchtop high- speed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of 7 fluorescent markers and two scatter parameters at a time."

• Beckam Coulter DU 520 UV/Vis spectrophotometer ( UV/vis spectrophotometer )
  

• Bio-Rad iCycler PCR ( PCR instrument )
  

• Gel doc ( Gel imaging system )
  

• Microplate washer ( Microplate washer )
  

• NuAire Nu-4750 CO2 incubator ( CO2 incubator )
  

  Lab contains four of these instruments.

• Real time PCR ( Real-time PCR machine )
  

• SD transfer cell ( Gel electrophoresis system )
  

• Thermo Scientific Well wash 888 Microplate washer ( Microplate washer )
  

Organisms and Viruses

• Dengue virus serotype 1 Hawaii ( Virus )
  

  Biological process studied: Pathogenesis and vaccine.
  Manifests disease: Dengue fever
  Model of disease: Rhesus

• Dengue virus serotype 2 Nueva Guinea ( Virus )
  

  Biological process studied: Pathogenesis and vaccine. Manifests disease: Dengue fever Model of disease: Rhesus

• Dengue virus serotype 3 ( Virus )
  

  Biological process studied: Pathogenesis and vaccine.
  Manifests disease: Dengue fever
  Model of disease: Rhesus

• Dengue virus serotype 4 ( Virus )
  

  Biological process studied: Pathogenesis and vaccine. Manifests disease: Dengue fever Model of disease: Rhesus

• Influenza virus S ( Virus )
  

  Environmental source: clinical
  Biological process studied: pathogenesis and vaccine
  Model of disease: Rhesus

• SIVsmE660 ( Virus )
  

  Biological process studied: vaccine
  Model of disease: Rhesus
  Environmental source: Laboratory adapted

• West Nile virus ( Virus )
  

  Strain: NY99
  Environmental source: Clinical
  Biological process studied: Pathogenesis
  Model of disease: Rhesus

Reagents

• 293T ( Tumor-derived cell line )
  

• BHK 21 cell line ( Tumor-derived cell line )
  

• BSC 1 cell line ( Tumor-derived cell line )
  

  Epithelial cells of African green monkey kidney origin.

• C6/36 cell line ( Cell line )
  

• LLC-MK2 cell line ( Tumor-derived cell line )
  

• MDCK cell ( Tumor-derived cell line )
  

• THP 1 cell line ( Tumor-derived cell line )
  

• Vero cell ( Tumor-derived cell line )
  

Services

• Dendritic cell immunophenotyping ( Material analysis service )
  

• ELISA ( Material analysis service )
  

• Florescence activated cell sorting service ( Material processing service )
  

• Immunofluoresence assay ( Material processing service )
  

• Polymerase chain reaction service ( Material processing service )
  

• RNA extraction ( Material processing service )
  

• Western blot analysis service ( Material analysis service )
  

Software

• Summit ( Software )
  

  Used with FACS Aria I instrument.

Web Links:

• http://cprc.rcm.upr.edu/units/virologylab