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|  University of Puerto Rico |
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| University of Puerto Rico |
The goal of the Infectious and Global Diseases Research Program (IGDRP) is to enhance the research capabilities for multidisciplinary collaborations that will lead to translational research in infectious and global diseases. The strategy to accomplish this goal is to provide access and support to advanced specialized technology that will enhance research capacity and promote activities that will improve interactions between clinicians and basic scientists and stimulate translational research. The IGDRP sponsors an “Infectious Diseases and Global Health Disparities Network” and a seminar series offered by worldwide recognized scientists to further enhance interactions between scientists working in infectious diseases within and outside Puerto Rico.
"This Bio safety Level 2 Tissue Culture Room is used to work with infectious agents. It is equipped with a Type II biological safety cabinet, water bath, CO2 incubator and autoclave."
"The TC10 automated cell counter provides a total count of mammalian cell and a live/dead ratio in one step."
"The Biorad Gel Doc system complemented with a charge coupled device (CCD) camera serve to capture images in real time, accurately position and focus the image and acquire photos of any type of gel. This system also has the Quantity One software to acquire images that can be optimized, annotate, analyze and print acquired pictures."
"The i-Cycler is also used to run PCR reactions, reverse transcription assays and ligation reactions."
"This microplate reader measures the absorbance of the contents in the wells of 96-well microtitration plates. It can perform single or dual wavelength measurements. This microplate can be controlled by the Microplate Manager Software program for protocol programming and data analysis."
"The CHEF-DR III system separates large and small DNA fragments. DNAs from 100 bases to over 10 megabases (mb) may be resolved."
"Designed to protect users during applications that emit vapors and/or chemical particles."
The 5810R centrifuge can be used to spin 1.5/2.0ml tubes, 15 ml and 50 ml tubes and 96 well plates. The temperature range of this centrifuge is from 9ºC to +40ºC.
"The Nikon E800 is a fixed-stage upright microscope configured to image in transmitted light, DIC, and epifluorescence modes. This microscope also has digital camera Spot RI and Metamorph software for pictures acquisition."
"The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format."
"The PCR workstation is used for the preparation of polymerase chain reactions using DNA and RNA. It contains a UV lamp for sterilization of the work area. "
"Shel Lab Refrigerated Incubator is a low temperature incubator ideal for testing B.O.D. of sewage and waste-water, as well as other low and room temperature applications."
"The SpectraMax M3 equipment is a versatile multimode system providing: UV-visible absorbance, fluorescence intensity and glow luminescence detections. This equipment has full spectral range detection for cuvettes and 96 well plates."
"For vapor sterilization of equipment, materials, or solutions (liquids)."
"The 96-well Veriti thermal cycler is used to run PCR reactions, reverse transcription assays and quick ligations. This equipment has a color touch-screen and intuitive interface, plus VeriFlex™ Blocks—six independent temperature blocks— for increased flexibility and precision."
"For routine incubation of Microplates and culture flasks the temperature range is from 25oC to 43oC."
"Clone Manager Software is a computer program used for in silico cloning (= cloning simulation), enzymatic digestions, sequence analysis, construct designs, operations and graphic map drawing. The user can also view or edit sequence files, find open reading frames, translate genes, or find genes or text in files."
"GraphPad Prism software is a program based in a combination of basic biostatistics, curve fitting and scientific graphing in one program (Data analysis (statistics) and graphics representations)."
"MetaMorph Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior."