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Fluorescence recovery after photobleaching protocol

eagle-i ID


Resource Type

  1. Protocol


  1. Resource Description
    "Fluorescence recovery after photobleaching (FRAP) denotes an optical technique capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies of cell membrane diffusion and protein binding. In addition, surface deposition of a fluorescing phospholipid bilayer (or monolayer) allows the characterization of hydrophilic (or hydrophobic) surfaces in terms of surface structure and free energy. Similar, though less well known, techniques have been developed to investigate the 3-dimensional diffusion and binding of molecules inside the cell; they are also referred to as FRAP." Reference: http://en.wikipedia.org/wiki/Fluorescence_recovery_after_photobleaching
  2. Additional Name
    FRAP protocol
  3. Uses
    Fluorescence recovery after photobleaching
  4. Uses
    Zeiss Axiovert 200 confocal microscope
  5. Uses
    Incubator INU-ZILCS-F1
  6. Used by
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
  3. created
  4. creator
    emiranda (Edgar Miranda)
  5. mediator
  6. modified

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016