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|  University of Puerto Rico |
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| University of Puerto Rico |
The DNA was isolated from peripheral blood using the QIAcube equipment. The gDNA was isolated and stored at -20⁰C.
The genetic material was isolated from Caribbean-Hispanic patients with Cardiovascular conditions, both females and males, of 21 years old and older.
The peripheral blood mononuclear cells comprise the following cells: B cells, T cells, NK cells, Monocytes and Dendritic cells. These cells were isolated by a density centrifugation, the differential migration during the centrifugation resulted in the formation of different layers. The layer of PBMC was found at the interface between the plasma and the density gradient medium. Therefore, this layer was isolated, washed and cryopreserved at -80⁰C.
These cells were isolated from Caribbean-Hispanic patients with Cardiovascular conditions, both females and males, of 21 years old and older.
The plasma was isolated after centrifugation of peripheral blood. The peripheral blood was collected using citrated-treated tubes. Plasma samples were isolated and stored at -80⁰C.
The peripheral blood was isolated from Caribbean-Hispanic patients with Cardiovascular diseases, both females and males, of 21 years old and older.
The database contains clinical information of Cardiovascular patients that are currently taking Plavix (clopidogrel) as part of their medical treatment. The patients are Caribbean Hispanics, females and males, of 21 years old and older. In this database, clinical information regarding comorbidities (e.g., diabetes, hypertension, dyslipidemias, etc.), co-medications, habits (e.g., smoking status), dosage and medical indications for Plavix, and prior history of cardiac interventions can be found
The database contains demographic data of Cardiovascular patients that are currently taking Plavix as part of their medical treatment. The patients are Caribbean Hispanics, females and males, of 21 years old and older. In this database, there is information regarding ethnicity, geographic location, age and gender, among others.
The database contains the genetic information of cardiovascular patients that are currently taking Plavix (clopidogrel) as part of their medical treatment. The patients are Caribbean Hispanics, females and males, of 21 years old and older. The genetic information that can be found in this database includes: Single Nucleotides Polymorphisms (SNPs) that might affect the response to Plavix in cardiovascular patients. In addition, there is information regarding the genetic ancestry of recruited patients in this database.
The database contains the laboratory test results of cardiovascular patients that are currently taking Plavix (clopidogrel) as part of their medical treatment. The patients are Caribbean Hispanics, females and males, of 21 years old and older. These test results are obtained from patient’s medical records or charts, and correspond to entries at their last medical appointment or during the day of recruitment. The collected data include: Hemoglobin, Hematocrit, Platelets count, White Blood Cells count, Serum Creatinine and Blood Urea Nitrogen (BUN), etc.
In this study, we want first to determine what genes are associated with clopidogrel response in Caribbean Hispanic patients and how the variability of these genes alter the effectiveness of clopidogrel treatment. The second part of this study is aimed at testing a method to guide the patient’s dual anti-platelet therapy (DAPT) using their clinical data, genetic test results and the results of a test to measure their platelet activity (PRU, using the VerifyNow equipment).
AirClean® Systems AC600 Series PCR Workstations combine ISO 5 HEPA-filtered air with UV light irradiation for the ultimate DNA/RNA manipulation and amplification work area. Cross-contamination during amplification of DNA and RNA can lead to results that are inaccurate, costing the lab technician valuable time and reagents. PCR Workstations minimize this risk by keeping airborne contaminants away from samples and irradiating work surfaces and tools between amplifications.
The LABGARD ES Model NU-425 Laminar Flow Biological Safety Cabinet (LFBSC) is a bench/table top model, optionally available with a base support stand, for operation as a console model. The LABGARD ES model NU-425 utilizes an Energy Saver DC ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and filter loading capacity. The Energy Saver ECM motor is controlled via a solid-state DC motor controller that provides automatic compensation (constant volume control) for both filter loading and line voltage variances. The NU-425 bench LFBSC is known as a Class II, Type A2 Biological Safety Cabinet.
The C-DiGit Blot Scanner is designed to image membranes for chemiluminescent Western blot analysis using digital methods, eliminating the need for traditional exposure to film. Before image acquisition is started, a membrane is placed on the scanning surface and the lid is closed. Image acquisition is started via Image Studio Software. The C-DiGit has one detection channel that detects visible wavelength emissions from chemiluminescent substrates. The C-DiGit Blot Scanner is a networkable device, and can be connected directly to a computer (Macintosh
through wired networks.
Eppendorf Centrifuge 5804 allows for molecular applications in tubes up to 250 mL and offers additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.
Rotor S–4–44
The Fisher Scientific Isotemp General Purpose Freezer fills your basic lab freezer needs. This steady laboratory upright freezer is efficient, and ideal for situations where extreme precision and control aren’t necessary. This 20.9 cu. ft. lab freezer has a temperature range of -12° to -20°C and uses 100% CFC-free R134a refrigerant with foamed in place polyurethane insulation. This single compartment lab freezer storage arrangement consists of 4 fixed height shelves, and one single hinged door with 6 shelves. The exterior is steel finished with an epoxy coating that resists corrosion and is easy to clean up with an ABS plastic interior.
Motic’s own CCIS maximizes optical clarity and scalability of the entire microscope. Future accessories or additions can be added without any detrimental effect on the optical system. The head is Binocular. The AE20 version Is equipped with WF 10X/20mm eyepieces giving an impressive wide field of view. The AE20 series Motic microscope features a class-leading 6V/30W quartz halogen illumination system providing bright and even illumination at any magnification.
Thermo Scientific™ NanoDrop 2000 is a full-spectrum, UV-Vis spectrophotometer used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000s measures sample volumes as small as 0.5µL. Micro-volume sample size (0.5-2.0µL). Easy to use—pipet sample directly onto the pedestal No dilutions required, even for highly concentrated samples. Fast measurement time of less than 5 seconds. Full spectral output (190 - 840 nm). Preconfigured methods for A260, A280, labeled DNA or Proteins, and much more. User-friendly software that includes custom methods and data export capabilities.
The ProFlex™ 96-Well PCR System allows you to: interchange the 96-well 0.2 mL block with another block type if your throughput needs change. Access the system remotely (and conveniently) through a mobile app. Program the instrument in seconds with a simple-to-use touchscreen interface. Simulate your old instrument with Thermal Simulation modes. Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex™ blocks.
The Fisher Scientific Sonic Dismembrator 100 is a great choice for the homogenizing, disruption cells, a mixing of small volumes of samples. This ultrasonic homogenizer transmits energy through a titanium probe for direct sonication into a liquid sample and creates cavitation. The Fisher 100 Sonic Dismembrator is designed for small samples, with a microtip probe and lower power output. This homogenizer processes samples up to 50ml and has an operating frequency of 23 kHz. Precise adjustment of this sonicator is controlled by the twist knob, and digital display.
Features of the The StepOne™ Real-Time PCR System include: Advanced software and instrumentation for performing a wide array of genomic assays. Sensitive 3-color optical LED recording system. Intuitive and robust software perfect for both first-time and advanced users. Simple and flexible instrument set-up and usage.
The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. Features; VeriFlex™ blocks to optimize PCR temperature with precision—the VeriFlex™ blocks provide 3 independent temperature zones, for precise temperature control over your PCR optimization. 8-inch color touch screen to help save time—the large and responsive color touch screen simplifies instrument operations with an easy to read interface. WiFi-enabled for remote monitoring—conveniently monitor your instrument from anywhere with our free PCR Essentials mobile app. Simple lid design to protect your sample—the innovative heated lid is easy to use and designed to help protect your samples. When open, it is in an upright position for easy handle access. Pull the handle forward to close the lid with ease. The heated lid will open automatically if not closed properly, to help safeguard your precious sample. Ready to use USB drive to easily back up your data—simply plug in a USB drive and follow the on-screen prompts to back up your data simply and effortlessly. Thermal simulation modes to replicate other PCR experiments—a library of simulation modes is available so that you may run an experiment using the same ramp rates as with a previous or different instrument.
The Eppendorf ThermoMixer C is for heating, cooling, and mixing almost any of your lab vessels. Reliable prevention of condensate formation in small vessels can be achieved with the heated lid Eppendorf ThermoTop. Features: mixing, heating, and cooling in one instrument for high flexibility; all common vessels and plate formats from 5 µL to 50 mL by different Eppendorf SmartBlocks™ for standard applications; excellent 2DMix-Control technology results in excellent sample mixing; anti-spill technology prevents lid wetting and cross-contamination; increased mixing frequency of up to 3,000 rpm for effective sample mixing; excellent temperature management for maximum temperature accuracy, resulting in safe handling; compatible with Eppendorf ThermoTop® (condens.protect®) for condensation-free sample handling (for Eppendorf SmartBlocks up to 2.0 mL); predefined program keys and clearly arranged menu guides enable simple and intuitive operations; freely programmable program keys for individual optimization; eppendorf PhysioCare Concept® provides ergonomic design and operation.
Thermo Scientific -80°C lab freezers are designed first and foremost to protect samples. They're also designed to maximize storage capacity by offering a range of upright and chest models to accommodate a variety of laboratory footprint requirements.
The VerifyNow System detects platelet activity by measuring in vitro platelet aggregation in a blood sample exposed to specific agonists. This includes inhibition of platelet activity in response to antiplatelet therapies. The VerifyNow® System is a whole blood, used in the laboratory or point-of-care setting, which measures platelet induced aggregation as an increase in light transmittance. It consists of a turbidimetric-based optical detection instrument, single-use test devices, and associated quality control. In general, platelet function testing measures the activity of platelets. Therapies that inhibit platelet function have
been used extensively to prevent the clinical complications of atherothrombosis. Three classes of potent antiplatelet agents, including acetylsalicylic acid(aspirin), P2Y12inhibitors (e.g. thienopyridines), and glycoprotein (GP) IIb/IIIainhibitors, have been developed.
The Applied Biosystems® Veriti® 96-Well Thermal Cycler delivers the proven reliability of Applied Biosystems® instruments. With the added control of VeriFlex™ technology, you have six independent temperature blocks that provide precise control over your PCR optimization. Additionally, the color touch screen simplifies setup and use of the Veriti™ 96-Well Fast Thermal Cycler. Optional setups for fast or standard PCR methods provide with flexibility to shorten your PCR cycling times. The Veriti™ 96-Well Thermal Cycler is equipped with a standard 0.2mL block configuration.